01
Standard library prep & PCR amplification.
Enabling NGS to do things it’s never done.
NGS is highly inefficient.
The applications of NGS remain limited by sequencing requirements. In oncology, most targeted molecules do not contain a variant of interest, resulting in the need to sequence vast numbers of molecules in each region.
Enspyre makes NGS 100x more efficient.
A revolutionary enrichment technology, Enspyre can remove >99% of the uninformative DNA from samples prior to sequencing in a simple, one-step addition to current hybridization capture workflows.
Increased accuracy from a fraction of the data.
By eliminating background DNA, Enspyre massively reduces the amount of data needed to answer genomic questions, providing increased accuracy from a fraction of the sequencing depth. It fits seamlessly into existing NGS workflows and can be applied across a wide range of applications.
Increased Efficiency
Sample enrichment provides 100x improvement in NGS efficiency
Seamless Integration
Simple integration into existing workflows & instruments
Compelling MRD Data
Ability to detect and quantify minimal residual disease (MRD) at gold-standard sensitivity from <3% of the sequencing depth of current approaches
How it Works
02
Hybridization capture of target regions by Enspyre bait probes. Off-target DNA removed by washing.
03
Pyrophosphorolysis digests only perfectly complementary bait probes.
04
Probe digestion releases mutated target sequences into supernatant. Beads and wild-type DNA are removed.
05
Supernatant is recovered and sequenced.
